|
KMID : 0043319860090030157
|
|
Archives of Pharmacal Research
1986 Volume.9 No. 3 p.157 ~ p.161
|
|
|
Fractionation of Enzymatically Methylated Acid-Insoluble Proteins from Thymus Nuclei
|
|
Lee HW
Kim SD/Paik WK
|
|
|
Abstract
|
|
|
|
Isolated calf thymus nuclei were in vitro methylated with S-adenosyl-L-methyl -14C methionine, and the proteins were fractionated according to their solubilities. Histone fraction(H2SO4-soluble fraction) contained approximately 60% total radioactivity incorporated, while "residual protein" which was H2SO4-insoluble contained the remaining radioactivity. The "residual protein" was further fractionated into various acidic proteins, which contained very little of the radioactivity. However, the protein fraction eluted from DEAE-cellulose with 0.5N NaOH contained the largest amount of radioactivity. This protein was found to be basic in nature by amino analysis.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|